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Stemline-Scetter- Index (SSI)
Take some data from the previous presentations in February. We have in several published papers created a parameter including the position about spredd of GI-coefficien of variation + adding of S-phase fraction at Feulgen stained tumor cells, end the spred of exceeding G2 cells based on image analysis These spred parameters: G1, S-phase and G2 fractions created together the Stemline-Scetter-Index (SSI) between <=6 to 6o relativ values.   Breast cancer and survival Screening of women’s breasts against cancer on regularly basis is very impotent for the outcum of cancer survival. Upp to Stemline–Scatter-Index to 12 – 15 mm tumors the result is very good. See next figuré.

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Triple Negative Breast Cancer (TNBC) poses a significant challenge due to its aggressive nature and lack of targeted therapy options. In the pursuit of novel treatment strategies, drug repurposing has emerged as a promising approach to expedite the drug development process. 2-Thio-6-azauridine, an antiviral drug with established safety profiles, has recently garnered attention for its potential in TNBC treatment. This abstract provides an overview of the journey of repurposing 2-thio-6-azauridine for TNBC therapy, highlighting its mechanism of action, preclinical studies, and potential clinical implications. Initially developed as an antiviral agent, 2-thio-6-azauridine demonstrates pleiotropic effects, including inhibition of RNA synthesis and disruption of nucleotide metabolism. Preclinical investigations have unveiled its ability to selectively target TNBC cells, exhibiting cytotoxicity and inhibitory effects on cell proliferation both in vitro and in vivo. Mechanistic studies elucidate its impact on key signaling pathways involved in TNBC progression, including the CD151 signaling. Moreover, synergistic effects have been observed when combining 2-thio-6-azauridine with conventional chemotherapy or targeted agents, suggesting potential combinatorial therapeutic strategies for TNBC management. Importantly, repurposing 2-thio-6-azauridine offers advantages such as accelerated clinical translation, reduced costs, and decreased risks associated with drug development. Moving forward, clinical trials are warranted to evaluate the efficacy and safety of 2-thio-6-azauridine as a monotherapy or in combination regimens for TNBC patients. Furthermore, biomarker-driven approaches may facilitate patient selection and optimize treatment outcomes. Overall, the repurposing of 2-thio-6-azauridine represents a promising avenue in the quest for effective and accessible therapies for TNBC, offering hope for improved survival and quality of life for patients facing this formidable disease.

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Some risks factors assotiated wuth COVID 19 infections were estableished such as coronary artery disease in patients with SARS-CoV-2 infections. The use of highly concentrated medications accorfing to Ardnt Shultz Law can induce reduction in the vital energy, leading to stagnation of Blood and propensity to have myocardail infaction even without arterail obstruction. Purpose; to demonstrate that patient with COVID 19 infection that received highly concentrated medications to treat this infection, have more propensity to  develop myocardila infarction some days after the treatment instituted. Methods; through one case report of 42 years-old patient with history of acquired COVID 19 on january 2nd 2021, he was admited in the hospital due to dyspnea symptons, myalgia, and needs oxigenation. He received the medications, ivermectine, hydroxicloroquine, corticosteroids . After 18 days of the initial manifestations, the patient felt pain in the chest and went to the hospital and they found that his troponin were increasing gradually and after two hours of it increased many times and the physician decided to admit him again in the hospital to make more exams. The patient was submitted to cattheterization and the result of this procedure were that all his coronary were in perfect state of health without any obstruction and they treated him as he had myocardial infaction. After three months , the patient went to the author´s clinic to evaluate his condition and she did chakras’ energy centers measurement.  Results: the results of this condition  that revealed that all his chakras’ were in the lowest level of energy with exception of the seventh that was normal. The author began his treatment with the use og homeopathies medications according to the theory Constitutional Homeopathy of the Five Elements based on Traditional Chinese Medicine. Conclusion; the conclusiopn of this study is that patients when treated with highlt concentrated medications to treat SARS-CoV-2 infection can develop myocadial ingaction without arterial obstruction due to energy deficiency state that agraveted many times due to the use of highly concentrrated medications used to treat this kind of infection nowadays.                  

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The human skin is the first layer of defense against external and environmental insults that increase the potential for developing skin cancer. Human skin is constantly exposed to two major environmental insults, namely solar UV radiation also known as simulated solar light (SSL), a known carcinogen, and pollutants such as benzo[a]pyrene (BaP), a suspected carcinogenic chemical which can be found in smoke originating from forest fires, cigarettes and burnt food. Due to the prevalence of these hazards in daily
life, it is important to understand the effects of BaP, SSL, and ultraviolet B (UVB) exposure on skin tissue, and the mechanisms by which BaP and UVB/SSL mediate their effects in order to design relevant strategies to mitigate their effects. Epidemiological studies indicate that increased exposure to these environmental hazards simultaneously can augment the risk of contracting human malignancies such as skin, mucosal, and lung cancers. Our previous studies demonstrated that exposure to UVB radiation
induces the generation of microvesicle particles (MVPs) from human and murine skin in a dose-dependent manner. Notably, our studies have also discovered that these MVPs carry a potent phospholipid mediator, Platelet-activating factor-receptor (PAFR) agonists. Importantly, these PAFR agonists play crucial roles in mediating the effects of pro-oxidative stressors, including UVB and therapeutic agents such as systemic immunosuppression and cancer growth in experimental models. To that end, our current studies sought to determine the effects of topical exposures to BaP, UVB, and SSL on MVP release from PAFR-expressing murine skin. The data demonstrated that these environmental hazards induce increased MVP release in a dose-dependent manner. While studies to determine the combined effects of BaP+UVB and BaP+SSL as well as the involvement of the PAFR and MVP are ongoing in the PAFR-deficient and acid sphingomyelinase enzyme deficient mice, the current studies are in agreement with this assumption that PAFR agonists-laden MVP release is one of the possible mechanisms in mediating these hazards-induced effects.

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Studies have demonstrated that cooking of meat at high temperature such as frying or barbeque causes production of heterocyclic amines (HCAs). At least a dozen of HCAs are found in cooked meat. The 2-amino-1-methyl-6-phenylimidazo[4-5-b]pyridine (PhIP) is abundant and most potent HCA in cooked meat. Several studies have shown that PhIP can induce tumors in breast, prostate and colon cells and in rodent models. It is shown that PhIP causes DNA mutation, promote tumor growth and promote invasiveness of cancer cells. PhIP metabolites are known to produce DNA adduct and DNA strand breaks. Phytonutrients are known to inhibit cytotoxic and genotoxic effects. Therefore, we hypothesized that the right combination of phytonutrients (naturally present in fruits, vegetables and spices) along with grilled meat should be capable of suppressing the PhIP induced cytotoxic actions.