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Day 1 full schedule

October 14, 2020 @ 10:00 - 17:20

Effects of Green Manure (Hairy vetch) Management Ways on Corn Growth and Grain Yield

Dr. Mohammad Zarif Sharifi

Dr. Mohammad Zarif Sharifi

Associate Professor

Afghanistan

ABSTRACT

     Corn is one of the three major cereals and the output has followed that wheat through the word. Especially in developing countries, corn grain is quite important as a food. Although corn production has been rapidly increased by the breeding, there are many cases in which the yield is low because of insufficient use of fertilizes in these countries, under such background, the practice of a crop rotation including leguminous green manure crop seems to be desirable.

     In this study nitrogen supplying capacity form hairy vetch (Vicia villosa Roth. HV) to corn was examined.

The experiment was carried out from 2017-2018 at the Research Farm of Faculty of Agriculture Kabul University, Kabul Afghanistan. Four treatments; incorporation of HV residue (B), mulching of HV residue on the inter-rows of corn (C), chemical fertilizer (D), and control (A), were set with three replications.

Incorporation of HV residue may be able to supply inorganic nitrogen of 130 ?    150 kg N ha -1 to the succeeding corn, since it was reported that 40 ?   50 % of HV nitrogen was mineralized in soil within 3 months after the incorporation.

     Above-ground dry matter (AGB) of the corn has always reached at 20 mg even in control, which was due to rather high soil fertility of the field, but it become significantly higher in both HV treatments, B and C, after the silky stage corresponding to the D plot. It was estimated that HV residue could supply 40% of nitrogen demand by corn. 

     These results designate that the nitrogen balance for corn production well be drastically improved by using HV cultivation in winter season.

The Halophyte Salicornia Bigelovii And The Innoculation With Plant Growth Promoting Bacteria: A Model Of Interaction Of Plant-Microorganism In Deserts

Dr. Edgar Omar Rueda Puente

Dr. Edgar Omar Rueda Puente

Research Professor

Mexico

ABSTRACT

 

The halophyte Salicornia bigelovii is vegetal with high potential to be improved in the deserts and sea costs of the northwest in México; however, its productivity depend of the nitrogen aportation, and others macro and micro nutrients essentials. Nitrogen-fixation by bacteria associated to the roots of Salicornia bigelovii and similar halophytes is an important source of available nitrogen in salt marsh ecosystems. However, the diversity of Salicornia’s rhizosphere is unknown. Areas in the volcanic zone of Sonora Desert and around the Bay of La Paz in Baja California Sur, Mexico were sampled to detect nitrogen-fixing bacteria associated to this halophyte: where 38 colonies were isolated. Two isolated microorganisms showed high acetylene reduction activity. The bacterium was identified as a Bacillus amilolyquefaciens and Klebsiella pneumoniae. Those bacteria, in conjunction with Azospirillum halopraeferens, were tested for growth-promoting ability when inoculated on S. bigelovii genotypes under several saline concentration conditions. During germination and early seedling growth, K. pneumoniae and Bacillus amilolyquefaciens showed high specificity. Also, the growth and development of the same two genotypes of S. bigelovii, were evaluated under field conditions. Bacillus amilolyquefaciens, Klebsiella pneumoniae and Azospirillum halopraeferens increased some growth and development parameters, using measures of weight, length of plants, and biochemical characteristics, including total protein, ash, and total lipid content in selected plant parts. The findings suggest that yields of both genotypes of S. bigelovii, under field conditions, can be improved by the application of Bacillus amilolyquefaciens, K. pneumoniae or A. halopraeferens strains. This is the first report of Bacillus amilolyquefaciens as nitrogen-fixing bacterium associated to the halophyte Salicornia bigelovii.

 

Comparative Transcriptome Profiling of Seed Ger mination Process in Two Contrasting Sodium Chloride Tolerance of Brassica napus L. Cultivars

Ibrahim A. A. Mohamed

Ibrahim A. A. Mohamed

PhD Scholar

China

ABSTRACT

Seedlings survival rate under salinity stress, vegetative growth, production yield and quality are highly related to seed germination and early growth performance. Germination of rapeseed cultivars is differing under NaCl stress, but the molecular mechanism of salinity in rapeseed poorly understood. In current study, we carried out integrative transcriptome analyses of four seed germination phases from two rapeseed cultivars under normal and NaCl stress conditions. The different pairwise comparisons of this study provides a comprehensive view of the dynamic biochemical processes that underpin salt tolerance and seed germination under salt and normal conditions. Our analysis identified DEGs for salt tolerance between the two cultivars were 5,325, 6,941, 5,835, and 9,114 down-regulated and 4,463, 4,710, 5,431 and 10,997 up-regulated in YSS/ZSS at 0h, 12h, 24h, and 48h, respectively. Furthermore, we identified the expression profiles and main pathways in each growth phase. For seed germination process, a considerable DEGs number, including those involved in “Pentose phosphate pathway”,  “Pyruvate metabolism“, “Protein processing in endoplasmic reticulum”, and “Glycolysis / Gluconeogenesis” pathways were specifically and transiently induced at the first 12 hours. “Photosynthesis”, “Carbon fixation in photosynthetic organisms”, “Amino sugar and nucleotide sugar metabolism”,  “Glyoxylate and dicarboxylate metabolism”, “Plant hormone signal transduction”, and “Glutathione metabolism” were enriched at the 48 hours.  In the comparison of salt tolerance between the two cultivars, the “plant hormone signal transduction”, “MAPK signaling pathway-plant”, and “starch and sucrose metabolism pathways were significantly enriched. More specifically, in the plant hormone signal transduction pathway, the genes regulated to Auxin signal transduction i.e. AUX1, TIR1, CH3 and SAUR exhibited significant upregulation that regulate cell enlargement and growth. Genes related to Cytokinine (AHP, and A-APR), Gibberellin (DELLA and TF), Brassinosteroid (BSK, BZR1/2, TCH4 and CYCD3), and Jasmonic acid (JAZ and MYC2) signal transduction were upregulated (Figure1). Our results provide new insights about transcript dynamics controlling plant hormone signal transduction pathway involved in the salt tolerance during the seed germination of rapeseed plants.

Exchange of sugarcane germplasm and knowledge of genomics is the present Worldwide needs for sweet revolution

Balwant Kumar

Balwant Kumar

Assistant Professor

India

ABSTRACT

Sugarcane has worldwide distribution for its cultivation and credited for ability to store

sweet in term of sucrose as human desire for sweet taste spans in all ages, races and cultures.  Throughout evolution, sweetness has been showed an important role and during prehistoric  times sugarcane cultivation has undergone many transformations into present-day noble cane. Domestication, selection of desirable clones and wide hybrids brought many agronomical useful traits into the cultivated species. Wild related species played a major role as the donor for most of the desirable qualitative and quantitative traits through gene introgression. Pre-breeding strategies, Intergeneric hybridization and ‘Nobilization’ have played a crucial role in development of noble high sugar cupled with  high-yielding canes. Cultivated sugarcane has been further enriched with other valuable traits such as high fiber, high sugar and tolerance to biotic and abiotic stresses. Despite its genomic complexity, crossability barriers within the genus, long breeding and selection cycles, etc., remarkable progress has been achieved to develop a wide range of cultivars, hybrids and mutants suitable for different agro-climatic conditions and some of them even stable up to 40-50 years. In a detail studies of ancestry of nine decade it was found that ‘Six high yielder with high sugared clones viz. POJ2878, Co285, Co281, CP28/11, Co213 & Co205 were mainly responsible for improvement in high yield and high sugar along with stable performance ability under North Central and North Eastern Zones of Sugarcane in India. Several research works were carried out at SRI, Pusa established in 1932, since last nine decade a sum total of 281 Sugarcane genotypes were developed by the institute among them 44 in the last decade. Altogether, 72 varieties were released by SRI, Pusa among them 47 were BO varieties while 25 comprises  Co & its allied varieties, after losing resistance against disease ‘red rot’, inferior juice quality with low yield potential varieties discarded/withdrawal from its commercial cultivation time to time. During evaluation of newly developed varieties viz,  ‘Evaluation of early maturing sugarcane clones’ - in spring season 2017 at research farm of RPCAU Pusa the clone CoP16437 showed highest value for cane yield and sugar yield followed by CoP15437 & CoSe12451. ‘Screening of variety under waterlogged’ -Four varieties viz. BO154, CoP092(CoP 09437), CoX07067 and BO155 were identified as water-logging tolerant during 2012-13. In ‘Evaluation of early maturing clone against red rot’ -The clones CoX 09726 significantly out yielded the check variety for both cane and sugar yield. The other clones CoX 09428, CoX 09715, CoX 09547, CoX 09647, CoX 09296 and CoX 09550 were statistically at par and also showed either resistant or moderately resistant reaction against red rot disease. In another trial ‘Evaluation of high sugared clone under water logged condition’- four clones namely CoX 12137, CoX 12164, CoX 12191 and CoX 12348 were selected as a water logging tolerant out of  eighteen sugarcane clones including two checks (BO 91 and CoLk 94184) at Rajendra Agricultural University, Pusa, Bihar in a RBD

with 3 replications during 2014 – 15 and it was also found that aerial roots on node of sugarcane clone play important role under water logged condition. In ‘Evaluation  of sixteen early maturing sugarcane for high cane and sugar yield’ -three Sugarcane Clones namely, CoP 15437, CoP 14436 and CoP 11437 were identified  as high  sugared coupled with  high cane yield bearing early maturing sugarcane during spring season 2016 these clones were planted at RPCAU, Pusa, farm  Bihar same year in another trial ‘to Evaluate Sixteen early maturing sugarcane clones’ and found  that six characters viz, cane yield, sugar yield, single cane weight, cane diameter, germination percentage at 90 days, shoots at 120 days as these characters showed high heritability coupled with genetic advance as per of mean. Well performance of the Clones namely, CoP 15437, CoP 14436 and CoP 11437 can be utilized for improvement of cane and sugar yield in early maturing sugarcane. In ‘Evaluation of early clones’ among the 15 test entries, the clone CoP16437 (Rajendra Ganna-1) showed highest value of cane yield and sugar yield followed by CoP15437 & CoSe12451 during crop season 17-18 at RPCAU,Pusa Farm, while in ‘Evaluation of thirteen mid late maturing sugarcane clones’ during 2018-19 at Research Farm, RPCAU, Pusa, Samastipur, Bihar a study on genetic variability, heritability and genetic advance for cane and  sugar yield along with its component traits. Highly significant differences for all the characters were observed among the 13 clones under studied. Higher numerical values of phenotypic variances and co-variances with respect to its genotypic counterpart were recorded for all the traits indicating greater environmental influence on these traits for total variation. High heritability coupled with high genetic advance as percent of means were observed for sugar yield, cane yield, germination % at 45 days, cane height, single cane weight at harvest and fiber % at harvest, it indicates the presence of additive gene action therefore, clonal selection based on above said traits might be effective method for sugarcane improvement programme. These traits can be considered for further clonal selection and genetic improvement of mid late maturing sugarcane clones. Germplasm collections, preservation and their utilization for development of an ideotype bearing desirable traits has become a research priority. For this purpose, molecular-marker tools are acting as potential drivers during pre-breeding and selection of desirable progenies. Supplementary tools such as in vitro culture, isolation of somaclones, induced mutagenesis and transgenics have played an essential role in the generation of novel genetic variability. In recent decades much emphasis has been given to enrich sugarcane breeding strategies; however, future avenues need to be focused on the utilization of inexhaustible and wealthy sugarcane genomic resources and advancements made through biotechnological interventions. As we know development of sugarcane clones and its recommendation for commercial cultivation as a variety takes 10-12 years through conventional breeding while use of genomics large scale hybrid clones can be  screen out within 2 years it saveed so much time up to 5 years. Earlier PoJ 2878 was exchanged as a result its most of the progenies after crossing  bearing  high sugar and popular under commercial cultivation. Since last five decade no such type of exchange of germplasm was done therefore improvement in term of genetic gain for sucrose content has been almost same as earlier. Brazil, India  Australia, USA, China, Thailand and several Sugarcane growing counties have one and more Research canters for development of sugarcane varieties and its gemplasm which needs to exchange and further nobilization. If improved variety have high sucrose than it will added more sugar even less coverage area.   Keeping all these above said observations Exchange of Sugarcane Germplasm and Knowledge of Genomics is the Present Worldwide Needs for Sweet Revolution.

Genetic diversity for grain nutrients content and interrelationship between nutrient and yield attributing traits in finger millet {Eleusine coracana (L.) Gaertn.} genotypes

Satish Kumar Singh

Satish Kumar Singh

Associate Professor

India

ABSTRACT

Finger millet is a promising source of micronutrients and protein besides energy and can contribute to the alleviation of iron (Fe), zinc (Zn) and protein malnutrition affecting women and preschool children in African and south east Asian countries. The most cost effective approach for mitigating micronutrient and protein malnutrition is to introduce staple crop cultivars selected or/ and bred for Fe, Zn and protein dense grain. Fifty genotypes of finger millet evaluated for genetic variability, heritability and genetic advance at research farm of Tirhut College of Agriculture, Dr. Rajendra Prasad Central Agricultural University, Pusa, Bihar. Data were recorded on five randomly selected plants per replication of each genotype for eleven quantitative characters, viz., iron content (mg kg-1 ), zinc content (mg kg-1 ), calcium content (g kg-1), protein (%),  plant height (cm), days to 50% flowering, number of basal tillers per plant,  (mm), ear head length (mm), ear head width (mm), fingers per ear head, grain yield (q/ha.). The genotypes exhibited highly significant differences for all the traits studied indicating sufficient genetic variability. Estimates of PCV and GCV for almost all the traits were nearly equal indicating the existence of immense inherent variability. The differences between the PCV and GCV estimates were relatively narrow for Ca and protein contents and large for Fe and Zn contents. High heritability for all the traits studied indicate that genotype play more important role than environment in determining the phenotype suggesting the predominance of additive gene action in the inheritance. The broad-sense heritability estimates were relatively higher for Ca and Protein and lower for Zn and Fe contents. The genotypes rich in Zn content have significantly higher grain yield potential than those rich in Fe and protein content. Grain nutrient specific genotypes were identified for use in the strategic research and cultivar development in finger millet.

Agronomic performance of early sugarcane genotypes to fertility levels under wider spacing

Navnit Kumar

Navnit Kumar

Assistant Professor

India

ABSTRACT

A field experiment was conducted during 2019 at Pusa, Bihar, to study the effect on seven sugarcane genotypes to different fertility levels at wider row spacing. Genotypes had significant impact on growth, yield and quality of sugarcane except single cane weight and purity percentage. Variety CoSe 95422 noticed maximum cane yield (102.6 t/ha) which was followed by CoSe 14454 (98.7 t/ha), CoSe 14451 (92.5 t/ha) and CoP 14437 (88.8 t/ha). Though, higher brix (21.6 %) and pol (18.92%) percentage juice was noticed due to the variety CoLk 94184 which was followed by CoSe 01421 and CoP 14437. Among fertility levels significantly higher plant population (1, 49,200/ha), millable canes (1, 07,300/ha) and cane yield (94.6 t/ha) was obtained with 125% recommended dose of NPK. Though, fertility level did not cause significant impact on brix, pol and purity percentage. Check variety CoSe 95422 was still doing best among the varieties. Application of 125% recommended dose of NPK was found superior with respect to plant population, millable canes and cane yield at wider row spacing.

Chloroplast DNA sequencing and analysis of three D-Genome cotton species

Farshid Talat

Farshid Talat

Assistant Professor

Iran

ABSTRACT

Cotton, as a world leading textile crop and a model system for studies of many biological processes, genomics research of cottons has advanced rapidly in the past few years. Gossypium contains five tetraploid (AD1 to AD5, 2n = 4X) and 47 diploid species (designated A through G, plus K, 2n = 2X), but the origin and evolution of allotetraploid Gossypium has remained controversial.

Complete chloroplast genome sequences belong to three diploid species were determined and annotated. Bioinformatics analyses showed that, the chloroplast genomes of Gossypium were highly conserved. Three chloroplast genomes were typical circular chromosomes like those of most other higher plants, including the large single copy (LSC), the small single copy (SSC) and two IR regions. The whole genome size ranged between 159,945 bp (G. laxum; D9), 159,973 bp (G. turneri; D10) and 160,122 bp (G. shwendimanii; D11). The assignment of the potential genes identified 140 genes for each genome, including 113 functional gene (79 of protein coding genes, 30 tRNA and 4 rRNA), 2 ORFs, 4 pseudogenes and 21 repeated genes. Four genes viz. infA, ycf68, ORF42 and ORF56, in addition to earlier mentioned genes, were been confirmed to be existed in studied genomes. SSRs totally varied from 62 to 64, and the average rate was 0.36 SSRs/kb between the three genomes.  This study revealed that wide ranges of expansions and contractions of IR are very common evolutionary events among 14 Gossypium species, which compared in our research. The phylogenetic analyses based on 50 protein-coding genes for 41 angiosperms and four gymnosperm out groups (Cycas, Ginkgo, Pinus and Gnetum) performed. Our phylogeny tree continued strongly support that Theobroma cacao as the closest species to Gossypium inside eudicots.

Role of rice lectin protein, Osr40c1, in imparting drought stress tolerance: A mechanistic approach

Riddhi Datta

Riddhi Datta

Assistant Professor

India

ABSTRACT

Lectin proteins are widely known to play key roles in various environmental stress responses in plants. Although the rice lectin protein, Osr40c1, has been reported to be regulated by drought stress, the molecular mechanism of how it imparts drought tolerance has not been explored so far. Our study has demonstrated that the expression of Osr40c1 gene positively correlates with the drought tolerance potential of various rice cultivars. Transgenic rice plants overexpressing the Osr40c1 displayed significantly improved tolerance to drought and salt stress over the wild-type plants. Interestingly, the protein displayed a nucleo-cytoplasmic localization and was  found  to  interact  with  a  number  of  drought-responsive  proteins  like OsSAM2, OsSAP8, OsMNB1B, and OsH4. Fascinatingly, the silencing of each of these protein partners led to drought susceptibility in the otherwise tolerant Osr40c1 expressing transgenic tobacco lines. This observation indicated that all these partner proteins were indispensable for the Osr40c1-mediated drought tolerance. In addition to this, the Osr40c1 protein was found to form a multi-protein complex with its partner proteins specifically under drought stress conditions. Together, our study revealed that the lectin protein, Osr40c1 imparts drought tolerance by  regulating  the  chromatin  proteins, OsMNB1B,  OsSAM2,  and OsH4, which presumably enables OsSAP8 to induce downstream gene expression.

Underutilized wonder plants of North East India and their ethno-medicinal uses

B.N. Hazarika

B.N. Hazarika

Professor

India

ABSTRACT

India is a part of both Himalaya as well as Indo-Burma biodiversity hotspots in the world. It has the richest reservoir of plant diversity in India and is one of the ‘biodiversity hotspots’ of the world supporting about 50 % of India’s biodiversity. Northeastern region occupy 7.7 % of total geographical area of country and harbours 50 % of Indian flora (8,000 species) of which about 4 % is endemic (2,526 species). The distinct tribes in the region have rich indigenous knowledge system on the use of components of biodiversity for their daily sustenance like food, fodder, shelter and healthcare. The region has several unique features such as fertile land, abundant water resources, evergreen dense forests of about 66%, high rainfall, and agriculture-friendly climate. Its unique phyto-geographical positions, topography and high degree of precipitation are some of the important factors which are mainly responsible for its enormous biological diversity. As a result, an array of wonder  plants are grown across the region ranging from tropical to alpine. A large number of diversity  in fruits belonging to the genera Artocarpus, Annona, Averrhoa, Garcinia, Musa, Passiflora, Phyllanthus, etc. are reported from the region. Besides diverse vegetables particularly wild leafy vegetables, rare genotypes of cucurbits, solanaceous vegetables, chilli, ginger, turmeric, etc. are there with some unique quality because of their locational advantage. The region has a great ethno-cultural diversity with major and sub-tribes, which explains the wealth of traditional ecological knowledge among farmers. People of region have their own culture, tradition and medicinal system of treatment and knowledge acquired through close observation of nature. Its ethnic people living in the remote forest areas still depend to a greater extent on the forest ecosystems for their livelihood They collect different medicinal plants and use them in traditional ways to cure their health related forms. The minor and wild fruits are mostly used to cure various gastrointestinal disorders, respiratory problems, cardiovascular compliance, muscular illness, bone diseases, gynaecological problem, cancers, snake bite, allergy and malaria etc. by local people of the region. This indigenous system of treatment based on such fruits is still an important part in social life and culture of the tribal people. However, this traditional knowledge of the local people has been transferred from generation to generation without proper technological interventions.

This paper provides the information on some of the wonder plants of the region and their ethno medicinal uses for the treatment of various health ailments by the tribes of  the region.

A Cellular Automata Framework for Modeling Octa-Filial Homozygosities of Crops, based on Allele encoding.

Praharshit Sharma

Praharshit Sharma

Member of BioClues Organization

India

ABSTRACT

In the realm of Plant Breeding and Genetics, Filial Generations are classified as F0, F1, F2, F3, F4, F5, F6, F7 and F8. Here, an attempt has been pioneered for Modeling the "Homozygosity" values for these Octa-Filials above based on a Elementary Cellular Automata (ECA) Framework, on the basis of Tripartite Symmetric Allele-encoding (Heterozygous, Homozygous Dominant/ Recessive). For instance, the Two "ECA-rules" namely, 191 and 247 exactly Model F2 and F5 Filial Generations, with Homozygosity values of 75% and 96.875% respectively. This is depicted in the IMAGE below. Interestingly, in such an ECA-based Octa-Filials Modeling Framework, the successive Filial Homozygosity values are found to be incremented by consecutively-Decreasing powers of 2, so as to say from (2^7=127) to (2^0= 1,unary offset) Additionally, a neat direct correlation is illustrated with the Generic "Breeder's equation" which is well characterized and quite familiar to the Plant Genomics Community worldwide. Furthermore, it is very interesting to make a striking Observation here, in this Context, that our Novel "ECA-Octa Filials" Framework can also accommodate for that matter, what can be construed as "Fractional Filials" (i.e,) intermediate Filial Generations as well, inbetween F0-F1, F1-F2, F2-F3, F3-F4, F4-F5, F5-F6, F6-F7 and F7-F8. Filials beyond Eight, such as F9 etc., may need different Automata Model

Speed Breeding: Tool to Enhance Crop Research

Praveen Kumar

Praveen Kumar

Assistant Professor

India

ABSTRACT

Generally, conventional breeding takes minimum 8 -10 years of breeding cycles to develop a new variety. Speed Breeding is a next generation advance technology where plants can be grown in an artificial environment with enhanced light duration to create longer daylight condition to speed up the breeding cycles of photo-insensitive crop. The speed breeding experiments in wheat revealed that the quality and yield of the plants grown under controlled climate with extended daylight were the same as those of crops grown in regular glass house conditions. The biggest problem of conventional breeding to achieve high yielding and more vigorous crop as it requires more generation to develop, but the speed breeding sorted out that problem. Farmers will have to produce 60 – 80 % more food by 2050 to feed 9 billion people and speed breeding is a potential technique to achieve it. Speed breeding, greatly shortens the generation time and accelerates breeding and research programmes. We can produce wheat generation from seed to seed in just a six week, which means we can do harvest of six generations per year. Plant pathogen interactions, plant anatomy and flowering time can be studied in detail and repeated using this technology. To respond faster to the changing climate, evolving pathogens a breakthrough technology in genetics and plant breeding programme should be needed and speed breeding will definitely full fill that in future. Shuttle breeding paves the way for screening of any diseases newly emerged across the countries. NASA is looking at ways to provide astronauts with nutrients in a long-lasting, easily absorbed form freshly grown fresh fruits and vegetables through speed breeding technology. As it increases the breeding generation per year, a greater number of researches will be carried out and documented.

Importance of Molecular study and its application for systematics and plant taxonomy

Mitra Lal Pathak

Mitra Lal Pathak

Sicentific Officer

Nepal

ABSTRACT

Molecular biology is a branch of biology that concerns the molecular basis of biological activity between biomolecules in the various systems of a cell including the interaction among DNA,  RNA, Protein and their biosynthesis as well as regulation of their interaction. Much of molecular biology is quantitative, and recently much work has been done at its interface with computer science in bioinformatics and computational biology. In the early 2000s, the study of gene structure and function, molecular genetics has been among the most prominent sub-fields. However this is developed now as as in fields in evolutionary biology such as population genetics and Phylogenetics.

The developed countries around the world, which accomplished their biological documentation very timely has been focusing on DNA or gene level (even whole genome) of each genus or species. The countries which has recently completed their flora or fauna has verifying the morphological work with molecular evidences. The developing or struggling countries in research are still struggling for flora and fauna records mainly based on morphology. However, sooner or later, our destination is same. Ultimately we need to verify or investigate all biological resources at molecular level for future research and their exact and proper utilization. So, this paper will emphasize how we can link molecular work for support of floral documentation of a country and what strategizes can be adopted ahead.

Genome-wide identification, characterization, and expression analysis of the dehydrin gene family in Asian pear (Pyrus pyrifolia)

Sayed Hussain

Sayed Hussain

Assistant Professor

Pakistan

ABSTRACT

Dehydrins (DHNs) are a complex family of plant proteins that play an important role in protection of higher plant cells from dehydration and desiccation damage during environmental stresses, such as drought, high salinity, and low temperature. However, information on DHN genes of Asian pear (Pyrus pyrifolia), an economically important fruit crop grown in temperate regions in East Asia, e.g., China and Japan, is limited. To gain insights into this gene family in pear and to elucidate their roles in floral buds under low-temperature conditions, we performed a genome-wide identification, characterization, and expression analysis of DHN genes. Seven PpDHN genes were identified. Sequence alignment analysis of all putative proteins from these genes showed that all of the proteins contained a typical K-domain. These genes were categorized into SKn,YnSKn,YKn, and Kn groups based on gene characterization and phylogenetic relationships. Hierarchical cluster analyses showed that in non-stressed pear, PpDHN genes were expressed in all vegetative tissues except young leaves and shoot tips, in which PpDHN1, PpDHN2, and PpDHN4were not expressed. Transcript levels of four PpDHN genes increased significantly in floral buds in response to low-temperature treatment, which indicated that they play important roles during stress adaptation. This study provides evidence that the family of pear DHN genes may will be valuable for further studies of the functions of DHN genes under different stress conditions in pear.